Co-inoculation of ryzobium strains and one of arbuscular mycorrhizial fungi (Glomus cubense) and its effect on kusdz? (Pueraria phaseoloides). Technical note

Resumen

The effect of co-inoculating two strains of ryzobium and one of arbuscular mycorrhizia fungi (AMF) Glomus cubense on the nutritional stage and yield of kusdz? (Pueraria phaseoloides vc. CIAT-9900), cultivated on a red lixiviated ferralitic soil, was studied. The experiment included eight treatments, distributing the ryzobium strains K1 and K2, the application of 50 kg of N ha-1, a control without ryzobium or nitrogen fertilizer, ryzobium alone or combined with G. cubense, in a random block design with four replicates. The results of the first cut are presented. In absence of the mycorrhizia inoculation, K2 produced N contents in the biomass (2.53 %) and yields of dry matter (2.73 t DM ha-1) significantly higher than K1, and similar to those reached with the nitrogen fertilization. However, the co-inoculation of G. cubense
with any of the ryzobium strains increased significantly the nodulation (46 nodules plant-1) and produced yields (3.37 t DM ha-1) and contents of N (3.35 %) and P (0.23 %) in the biomass significantly higher than those reached with the ryzobium alone or with the application of N. The fungal structures of the plants were also favored with the combination of the ryzobium and the AMF. Under the conditions of this experiment, the synergic effect of both microorganisms was proved, as well as the advantages of their co-inoculation to improve yield and nutritive value of kusdz? at least during its establishment.
Key words: Rhizobium, Glomus cubense, forage legumes, nitrogen biological fixation, nutrition.