Selection of rhizobia isolates from nodules of the forage legume Pueraria phaseoloides (tropical kudzu)
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Abstract
of tropical kudzu in livestock production soils. Their possible taxonomic distribution and their capacity of growing in six carbon sources and produce polyhydroxybutyrates, were determined. Also, in vitro nodulation bioessays were conducted in the host plant. A completely randomized design with ten observations per treatment was used. All isolates resulted possible members of the Bradyrhizobium genus. Six of them consumed all the sugars as only carbon source, galactose was the least consumed.? Polyhydroxybutyrate concentrations produced by these isolates were between 0.001 and 0.009 g.L-1, what was significantly higher than the rest of the isolates. Plants inoculated with two of the isolates showed greater aerial dry mass which tallies with those having greater effective nodulation. From the ten isolates, four showed greater potentialities for the preparation of an effective inoculant for tropical kudzu culture.
Key words: identification, biofertilizer, sugars, PHB, nodulation
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