Genetic diversity of the Landim mozambicano goat

Main Article Content

A. Cavele
E. Pérez Pineda
N. Fonseca
J. L. Ramírez
D. Verdecia
María A. Martínez
J. V. Delgado
C. J. Barba
J. Grizelj

Abstract

With the purpose of determine the genetic diversity of the "Landim Mozambicano" goat, a panel of 33 microsatellites molecular markers were analyzed, selected and recommended by the “Comité de Expertos de la FAO” and the “Sociedad Internacional de Genética Animal” for diversity studies in sheep and goats species. Hair samples of 60 individuals from six Mozambique provinces (Manica, Tete, Sofala, Nampula, Gaza e Inhambane) were taken, which groups the highest number of animals from the breed. The ADN of the capillary bulb was extracted and amplified by PCR in the laboratorio de Biología Molecular del Animal Breeding Consulting, S.L, de la Universidad de Córdoba, España. Electophoresis in poliacrilamide gel was carried out to separate the obtaining fragments in an Automatic Sequencer ABI Prism® 377 XL (Applied Biosystems).The fragments analysis and genotyped was performed by the informatics programs Genescan and Genotyper 3.7 NT. It was determined: allele total per marker, expected and observed heterozygosities and Polymorphic Information Content. All the microsatellites were polymorphic, a total of 214 alleles with 6.4 as average per marker were found. From the analyzed panel a total of 29 were informative, 11 highly informative and 3  exceptionally informative for the breed. As main find, the heterozygosities reached values higher than 60 %. It is concluded that the microsatellites battery was adequate to study the breed, four of them can be excluded in future studies and the "Landim" goat is a breed with adequate genetic diversity.


Key words: goats, markers, ADN, Mozambique.

Article Details

How to Cite
Cavele, A., Pérez Pineda, E., Fonseca, N., Ramírez, J. L., Verdecia, D., Martínez, M. A., Delgado, J. V., Barba, C. J., & Grizelj, J. (2023). Genetic diversity of the Landim mozambicano goat. Cuban Journal of Agricultural Science, 57. Retrieved from https://cjascience.com/index.php/CJAS/article/view/1087
Section
Animal Science

References

Al-Atiyat, R.M., Alobre, M.M., Aljumaah, R.S. & Alshaikh, MA. 2015. "Microsatellite based genetic diversity and population structure of three Saudi goat breeds". Small Ruminant Research, 130: 90-94, ISSN: 0921-4488. https://doi.org/10.1016/j.smallrumres.2015.07.027.

Aranguren M.J., Portillo, R.M., Rincón, X., Amparo, M.M., Dickson, L.A. & D’aubetere, R. 2013. "Diversidad genética en la cabra criolla venezolana mediante análisis con microsatélites". Revista Científica, XXIII (3): 238-244, ISSN: 0798-2259.

Arellano, H., Mascorro, G., Bribiesca, E., Hernández, G., De la Cruz, C. & López, S. 2020. "Morphostructural variability in the Pastoreña goat in different regions of the Mixteca of México". Rev. FCA UNCUYO. 52(2): 360-375. ISSN 1853-8665.

Bosman, L., Van Marle-Köster, E. & Visser, C. 2015. "Genetic diversity of South African dairy goats for genetic management and improvement". Small Ruminant Research, 123(2-3): 224-231, ISSN: 0921-4488. https://doi.org/10.1016/j.smallrumres.2014.12.003.

Botstein, D., White, R.L., Skolmick, H. & Davis, R.W. 1980. "Cosntruction of genetic linkage map in man using restiction fragment lenght polymorphism". American Journal of Human Genetics, 32:314-331. ISSN: 1537-6605

Bustamante, C. 2019. Determinación de la diversidad y estructura genética de la cabra criolla (Capra hircus Linnaeus) de los departamentos de Lima y Piura mediante el uso de microsatélites. Tesis para optar el título profesional de Biólogo con mención en Hidrobiología y Pesquería. Escuela Profesional de Ciencias Biológicas, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.

Chacón, M.E., La O, A.M., Fonseca, F.N., Pérez Pineda, E., Velázquez, R.F., Coss, D.Y., Fonseca, J.Y., Delgado, J.V. & Martínez, M.A. 2017. Caracterización genética y conservación de la cabra criolla cubana. En: Biodiversidad Caprina Iberoamericana. Cuellar, C. Ed. Ediciones Universidad Cooperativa de Colombia. Bogotá, Colombia, p. 75. ISBN 978-958-760-067-4.

Chacón, M.E., Martínez, M.A., Pérez Pineda, E., Delgado, J.V., Fonseca, J.Y. & Yusel, C.D. 2018. Conservación de la cabra Criolla Cubana a partir de estudios de su diversidad genética. V Simposio Internacional de Producción Animal Guayaquil. 2018, Guayaquil, Ecuador.

De Sousa, B.C., Martínez, M.A., Ginja, C.., Santos-Silva, F., Carolino, M.I., Delgado, J.V. & Gama, L.T. 2011. "Genetic diversity and population structure in Portuguese goat breeds". Livestock Science, 135(2-3): 131–139, ISSN: 1871-1413. https://doi.org/10.1016/j.livsci.2010.06.159.

Du, X., Cao, J., Han, X., Hao, H., Yu, M. & Zhang. G. 2017. "Genetic diversity and population structure among eight Chinese indigenous goat breeds in the Yellow River valley". Small Ruminant Research, 148: 87-92, ISSN: 0921-4488. https://doi.org/10.1016/j.smallrumres.2016.12.034.

FAO. 2011. Molecular genetic characterization of animal genetic resources. FAO Animal Production and Health Guidelines. No. 9, 85 pp. ISBN: 978-92-5-107032-1. Rome, Italy.

FAO. 2015. The Second Report on the State of the World’s Animal Genetic Resources for Food and Agriculture. Rome, Italy.

Garrine, C., Kotze, A., Els, H. & Grobler, J. 2010. "Genetic characterization of the indigenous Landim and Pafuri goat breeds from Mozambique". African Journal of Agricultural Research, 5(22): 3130-3137, ISSN: 1991-637X. https://doi.org/10.5897/AJAR.

Ginja, C., Gama, L.T., Martínez, M.A., Sevane, N., Martínez‐Burrel, I., Lanari, M.R., Revidati, M.A. Arangueren‐Méndez, J.A., Bedoti, D.O., Ribeiro, M.N. & Sponenberg, P. 2017. "Genetic diversity and patterns of population structure in Creole goats from the Americas". Animal Genetics, 48(3): 315-329, ISSN: 1365-2052. https://doi.org/10.1111/age.12529.

Gómez, N.C. 2013. Caracterización morfológica y faneróptica del caprino apurimeño peruano. Tesis en opción al grado científico de Doctor en Ciencias Veterinarias. Universidad Autónoma de Barcelona. España.

Hussaint, T., Babar, M., Sadia, H., Shaheen, M., Nadeem, A. Ali, A. & Shah, S. 2013. "Microsatellite markers based genetic diversity analysis in Damani and Nachi goat breeds of Pakistan". Pakistan Veterinarian Journal, 33(4): 520-522, ISSN: 2074-7764.

Jiménez, A.P., Bedoya, J., Serrano, C., Arcila, V., Pérez, V., Serrano, L.K., Ascanio, C.A., Sánchez, L.A. & Malpica, M.E. 2014. "Diversidad genética de la cabra santanderena mediante marcadores microsatéllites". Actas Iberoamericanas de Conservación Animal, 4: 105-107, ISSN: 2253-9727.

Martínez, A.M. 2001. Caracterización genética del cerdo Ibérico mediante marcadores moleculares. Tesis doctoral. Universidad de Córdoba. Córdoba. España.

Murital, I., Afolayan, O., Bemji, M.N., Dadi, O., Landi, V., Martínez, M.A., Delgado, J.V., Adebambo, O.A., Aina, A.B.J. & Adebambo, A.O. 2015. "Genetic diversity and population strutrure og Nigerian indigeneus goat using DNA microsatellite markers". Archivos de Zootecnia, 64 (246): 93-98. ISSN: 0004-0592.

Nei, M. 1973. Analysis of gene diversity in subdivided population. Proceedings of the National Academy of Science. USA. 70: 3321-3323.

Nhacumbe, A., Cavele, A., Cala, A., Uzêda, R., Souza, B., Gondim, L., Andrade, M., Almeida, M., de Matos, C. & Maricoa, N. 2017. "Serological survey of Neospora caninum and Besnoitia besnoiti in cattle and goats from smallholder farms in Angónia, Tete Province, Mozambique". African Journal of Rural Development, 2(2): 303-311, ISSN: 2814-0346. https://doi.org/10.22004/ag.econ.263576.

Ott, J. 1992. "Strategies for characterizing highly polymorphic markers in human gene mapping". American Journal of Human Genetics, 51(2): 283-290, ISSN: 0002-9297.

Raghavendra, C. 2016. Genetic characterization of Mahabubnagar goats based on microsatellite markers. Master Thesis. Narasimha Rao Telangana State University, India.

Raymond, M & Rousset, F. 1996. "An exact test for population differentiation". Evolution, 49:1280-1283. ISSN:1558-5646.

Silva, JC., Andrade, M.H., Vera, A.H., Marina, D.A. Román, S.I., Landi, V. Martínez, M.A., Delgado, J.V. & Consorcio BioGoat. 2019. "Diversidad y estructura genética de una población de cabras criollas negras de tres municipios del estado de Querétaro, México". Revista Mexicana de Ciencias Pecuarias, 10(4): 801-818, ISSN: 2007-1124. https://doi.org/10.22319/rmcp.v10i4.4908.

Skapetas, B. & Bampidis, V. 2016. "Goat production in the world: present situation and trends". Livestock Research for Rural Development, 28: 200, ISSN: 2521-9952.

Sulabda, N., Susari, N., Heryani, N. & Puja, I. 2012. "Genetic diversity of Gembrong goat based on DNA microsatellite markers". Indonesian Journal of Animal and Veterinary Science, 17: 25-35, ISSN: 2722-421X.

Vaiman, D., Schibler, L., Bourgeois, F., Oustry, A., Amigues, Y. & Cribiu, E.P. 1994. "A genetic linkage map of the male goat genome. Genetics, 144(1): 279-305. PMC1207502.

Vahidi, S.M.F., Tarang, A.R., Naqvi, A.N., Falahati Anbaran, M., Boettcher, P. & Joost, S. 2014. "Investigation of the genetic diversity of domestic Capra hircus breeds reared within an early goat domestication area in Iran". Genetics Selection Evolution, 46: 1-12, ISSN: 1297-9686.

Villarreal-Arellano, H.R., Fuentes-Mascorro, G., Ramírez-Bribiesca, J.E., Torres-Hernández, G., Ricardi-De-la-Cruz, C. & Vargas-López, S. 2020. "Morphostructural variability in the Pastoreña goat in different regions of the Mixteca of México". Revista de la Facultad de Ciencias Agrarias UNCUYO, 52(2): 360-375, ISSN: 1853-8665.

Most read articles by the same author(s)

<< < 1 2