Genetic characterization of the Cuban Creole goat through microsatellite markers
Barra lateral del artículo
Publicado:
sept. 1, 2010
Contenido principal del artículo
Resumen
Samples of hair from 40 goats were collected to characterize genetically the Cuban Creole goat as part of the research project for the
preservation and improvement of this breed. Out of them, 33 % came from the only genetic nucleus of this breed in Cuba, located in the
Genetic Enterprise ?Manuel Fajardo? in Jiguan?, Granma. The rest of the goats were from several farms, cooperatives of agricultural production, and other herds from the provinces of Granma, Santiago de Cuba, and Holgu?n. A set of 26 microsatellites was used, as recommended by the FAO and the International Society of Animal Genetics (ISAG) for studies of genetic diversity in the goat species. The amplification of them was performed through the polymerase chain reaction (PCR) and the amplified fragments were separated by electrophoresis in polyacrylamide gels. The allelic frequencies, the expected (He) and the observed (Ho) heterozygosities, and the polymorphic information content (PIC) were calculated, as well as the Hardy-Weinberg equilibrium (HWE) per marker. The mean value of the alleles per locus was of 6.38. the totality of the loci under study were polymorphic in this breed, and 77 % of them showed high polymorphic information content (PIC). This, along with the presence of more than 58 % of heterozygotic individuals, evidences that the Cuban Creole goat is a breed with self-identity having high genetic diversity.
Key words: Creole goats, polymorphism, microsatellites.
preservation and improvement of this breed. Out of them, 33 % came from the only genetic nucleus of this breed in Cuba, located in the
Genetic Enterprise ?Manuel Fajardo? in Jiguan?, Granma. The rest of the goats were from several farms, cooperatives of agricultural production, and other herds from the provinces of Granma, Santiago de Cuba, and Holgu?n. A set of 26 microsatellites was used, as recommended by the FAO and the International Society of Animal Genetics (ISAG) for studies of genetic diversity in the goat species. The amplification of them was performed through the polymerase chain reaction (PCR) and the amplified fragments were separated by electrophoresis in polyacrylamide gels. The allelic frequencies, the expected (He) and the observed (Ho) heterozygosities, and the polymorphic information content (PIC) were calculated, as well as the Hardy-Weinberg equilibrium (HWE) per marker. The mean value of the alleles per locus was of 6.38. the totality of the loci under study were polymorphic in this breed, and 77 % of them showed high polymorphic information content (PIC). This, along with the presence of more than 58 % of heterozygotic individuals, evidences that the Cuban Creole goat is a breed with self-identity having high genetic diversity.
Key words: Creole goats, polymorphism, microsatellites.
Detalles del artículo
Cómo citar
Chac?n, E., Mart?nez, A., La O, M., Vel?zquez, F. J., P?rez, E., & Delgado, J. V. (2010). Genetic characterization of the Cuban Creole goat through microsatellite markers. Cuban Journal of Agricultural Science, 44(3). Recuperado a partir de https://cjascience.com/index.php/CJAS/article/view/212
Sección
Genética
Aquellos autores/as que tengan publicaciones con esta revista, aceptan los términos siguientes:
- Los autores/as conservarán sus derechos de autor y garantizarán a la revista el derecho de primera publicación de su obra, el cuál estará simultáneamente sujeto a la Licencia Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) que permite a terceros compartir la obra siempre que se indique su autor y su primera publicación esta revista. Bajo esta licencia el autor será libre de:
- Compartir — copiar y redistribuir el material en cualquier medio o formato
- Adaptar — remezclar, transformar y crear a partir del material
- El licenciador no puede revocar estas libertades mientras cumpla con los términos de la licencia
Bajo las siguientes condiciones:
- Reconocimiento — Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace.
- NoComercial — No puede utilizar el material para una finalidad comercial.
- No hay restricciones adicionales — No puede aplicar términos legales o medidas tecnológicas que legalmente restrinjan realizar aquello que la licencia permite.
- Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
- Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos telemáticos institucionales o en su página web) antes y durante el proceso de envío, lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).