Effect of Aspergillus oryzae on the rumen fermentation of Pennisetum purpureum cv. Cuba CT-115 through the in vitro gas technique
Article Sidebar
Main Article Content
Abstract
with two assays was performed according to completely randomized design, applying the in vitro gas production technique. Five treatments
were assessed. In the first stage of the experiment, the doses of 0, 500, and 1000 ?L of culture of A. oryzae in 80 mL of incubation solution were compared. In the second, 0, 100, and 200 ?L of culture in 80 mL of incubation solution were compared. There were four repetitions per treatment. In the first stage, the readings were performed every three hours until 24 h of incubation, and, in the second, every two hours until 77 h. The parameters of the kinetics of fermentation were determined in the doses of 0, 100, and 200 ?L. The results showed that the adequate dose of microbial additive was 200 ?L of culture in 80 mL of incubation solution. The values of gas production with this level of inclusion were higher (P < 0.001) than those of the control without inoculum. This dose showed higher potential of gas production, higher speed of fermentation, and lower lag phase, compared with the control without inoculum. The levels of 500 and 1000 ?L of culture of A. oryzae provoked a decline in gas production. It is suggested that the addition of this strain of A. oryzae in doses of 200 ?L of culture in 80 mL of incubation solution is able of stimulating the rumen fermentation of Pennisetum purpureum under in vitro experiments.
Key words: microbial additives, Aspergillus oryzae, gas production, roughages.
Article Details
Those authors that have publications with this journal accept the following terms:
1. They will retain their copyright and guarantee the journal the right of first publication of their work, which will be simultaneously subject to the License Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) that allows third parties to share the work whenever its author is indicated and its first publication this journal. Under this license the author will be free of:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
2. The authors may adopt other non-exclusive license agreements to distribute the published version of the work (e.g., deposit it in an institutional telematics file or publish it in a monographic volume) whenever the initial publication is indicated in this journal.
3. The authors are allowed and recommended disseminating their work through the Internet (e.g. in institutional telematics archives or on their website) before and during the submission process, which can produce interesting exchanges and increase the citations of the published work. (See the Effect of open access).