Methodology for the isolation, identification and selection of Bacillus spp. strains for the preparation of animal additives
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Abstract
chloride and bacteriological peptone); the third stage included the integral evaluation of the effect of animal additives on probiotic response indicators in birds. With the proposed methodology, 100 strains were isolated, 48 of them with characteristics of the Bacillus spp. genus. The three strains were characterized (C-31, C-34 and E-44) that had the best characteristics as candidates for probiotics, capable of manifesting high growth capacity (P <0.001) in the new culture medium. The production cost of them was 2.34 Cuban pesos and 0.54 / L USD. These three animal additives showed their effect on chickens, when improving the probiotic
response in the microbiological, immunological, fermentative, hematological and productive indicators in these animals. From the
obtained results, it can be assured that the methodological proposal to isolate, identify and select Bacillus spp. strains can be applied in Cuba to improve the productive response in livestock interest.
Key words: methodology, probiotics, bacilli
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