Genetic characterization of the Cuban Creole goat through microsatellite markers
Article Sidebar
Main Article Content
Abstract
preservation and improvement of this breed. Out of them, 33 % came from the only genetic nucleus of this breed in Cuba, located in the
Genetic Enterprise ?Manuel Fajardo? in Jiguan?, Granma. The rest of the goats were from several farms, cooperatives of agricultural production, and other herds from the provinces of Granma, Santiago de Cuba, and Holgu?n. A set of 26 microsatellites was used, as recommended by the FAO and the International Society of Animal Genetics (ISAG) for studies of genetic diversity in the goat species. The amplification of them was performed through the polymerase chain reaction (PCR) and the amplified fragments were separated by electrophoresis in polyacrylamide gels. The allelic frequencies, the expected (He) and the observed (Ho) heterozygosities, and the polymorphic information content (PIC) were calculated, as well as the Hardy-Weinberg equilibrium (HWE) per marker. The mean value of the alleles per locus was of 6.38. the totality of the loci under study were polymorphic in this breed, and 77 % of them showed high polymorphic information content (PIC). This, along with the presence of more than 58 % of heterozygotic individuals, evidences that the Cuban Creole goat is a breed with self-identity having high genetic diversity.
Key words: Creole goats, polymorphism, microsatellites.
Article Details
Those authors that have publications with this journal accept the following terms:
1. They will retain their copyright and guarantee the journal the right of first publication of their work, which will be simultaneously subject to the License Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) that allows third parties to share the work whenever its author is indicated and its first publication this journal. Under this license the author will be free of:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
2. The authors may adopt other non-exclusive license agreements to distribute the published version of the work (e.g., deposit it in an institutional telematics file or publish it in a monographic volume) whenever the initial publication is indicated in this journal.
3. The authors are allowed and recommended disseminating their work through the Internet (e.g. in institutional telematics archives or on their website) before and during the submission process, which can produce interesting exchanges and increase the citations of the published work. (See the Effect of open access).