Stability of the zootechnical additives SUBTILPROBIO® C-31, C-34 and E-44 under different temperature conditions
Article Sidebar
Main Article Content
Abstract
In intensive livestock production, not all storage conditions are available for the conservation of biological products. Hence, the objective of this research was to verify the stability of three zootechnical additives: SUBTILPROBIO® C-31, C-34 and E-44 at room temperature (24 ± 3 ºC) and in refrigeration (4 ± 8 ºC). For this, three experiments were carried out: 1) microbiological quality of the additives, 2) viability of the endospores and 3) pH dynamics. The results revealed the absence of contaminating microorganisms in the additives, such as fecal and total coliforms, Pseudomonas auruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella. The count of yeasts (<103mL) and fungi (<10CFU.mL-1), acceptable for products of animal consumption. The viability of endospores showed no difference in both temperatures (16 Log CFU.mL-1). However, the pH showed differences (P <0.05) between the initial hour and the seventh day for both temperatures [environment: C-31: 6.38 / 5.70; C-34: 6.38 / 5.74; E-44: 6.38 / 5.77 and refrigeration: C-31: 6.38 / 4.52; C-34: 6.38 / 4.50; E-44: 6.38 / 4.49]. It can concluded
that there is no difference for both temperatures, which makes viable the preservation of zootechnical additives SUBTILPROBIO® C-31, C-34 and E-44 in both conditions.
Key words: conservation of biological products, Bacillus subtilis,
viability of endospores
Article Details
Those authors that have publications with this journal accept the following terms:
1. They will retain their copyright and guarantee the journal the right of first publication of their work, which will be simultaneously subject to the License Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) that allows third parties to share the work whenever its author is indicated and its first publication this journal. Under this license the author will be free of:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
2. The authors may adopt other non-exclusive license agreements to distribute the published version of the work (e.g., deposit it in an institutional telematics file or publish it in a monographic volume) whenever the initial publication is indicated in this journal.
3. The authors are allowed and recommended disseminating their work through the Internet (e.g. in institutional telematics archives or on their website) before and during the submission process, which can produce interesting exchanges and increase the citations of the published work. (See the Effect of open access).