Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana
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Abstract
oryzae on the ruminal microbiota and on some end products of the fermentation. The trial was conducted according to completely randomized design, with factorial fit 4 x 3 for the microorganisms, and 4 x 6 for the end products of the fermentation. The treatments consisted of a control, without inoculation, and three ways of inclusion of the additive: fungus culture, biomass without culture medium and culture broth. Three repetitions were conducted per treatment. The concentrations of total anaerobic, cellulolytic and proteolytic bacteria, fungi and protozoa, total short chain fatty acids (SCFAt), ammonia and ruminal pH were determined as fermentative indicators. Samples were collected to determine the microbial populations at 0, 3 and 6 h, and for the pH and the end products of the fermentation at 0, 3, 6, 9, 12 and 24 h. The populations of total anaerobic, cellulolytic bacteria and fungi were increased with the addition of this additive. The concentration of SCFAt increased 1.66 folds and the levels of NH3 diminished. In respect to the proteolytic bacteria, the protozoa and the pH, no differences were found compared with the control without inoculation. It is concluded that the addition of Aspergillus oryzae in the culture medium favors the in vitro ruminal fermentation of Pennisetum purpureum cv. Cuba CT-115, by increasing certain microbial populations and the concentration of SCFAt.
Key words: microbial additives, Aspergillus oryzae, discontinuous culture of rumen microorganisms, roughages.
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