Effect of Aspergillus oryzae on the rumen fermentation of Pennisetum purpureum cv. Cuba CT-115 through the in vitro gas production technique

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Areadne Sosa
Juana Galindo
Ram?n Bocourt
R. Rodr?guez
Nereida Albelo
A. Oramas

Abstract

In order to determine the effect of Aspergillus oryzae on the rumen fermentation of Pennisetum purpureum cv Cuba CT-115, an experiment was conducted with two trials, according to complete random design using the in vitro gas production technique. Five treatments were assessed. The doses of 0, 0.5, and 1 mL of A. oryzae culture in 80 mL of incubation solution were compared in the first stage, and those of 0, 0.1, and 0.2 mL culture in 80 mL of incubation solution were compared in the second. Four repetitions were carried out per treatment. In the first trial, the readings were performed every 3 h up to 24 h of incubation and, in the second, every two hours up to 77 h. The parameters of the fermentation kinetics were determined in the doses of 0, 0.1, and 0.2 mL. The outcomes showed that the adequate dose of microbial additive was of 0.2 mL of culture in 80 mL of incubation solution. The values of gas production attained with this level of inclusion were higher (P < 0.001) than those of the control without inoculum. This dose showed higher potential of gas production, higher fermentation speed, and lower lag phase, compared with the control without inoculum. The levels of 0.5 and 1 mL of A. oryzae culture provoked a decline in gas production. It is suggested that the addition of this strain of A. oryzae at doses of 0.2 mL of culture in 80 mL of incubation solution is able of stimulating the rumen fermentation of Pennisetum purpureum in experiments under in vitro conditions.
Key words: microbial additives, Aspergillus oryzae, gas production, roughages.

Article Details

How to Cite
Sosa, A., Galindo, J., Bocourt, R., Rodr?guez, R., Albelo, N., & Oramas, A. (2010). Effect of Aspergillus oryzae on the rumen fermentation of Pennisetum purpureum cv. Cuba CT-115 through the in vitro gas production technique. Cuban Journal of Agricultural Science, 44(1). Retrieved from https://cjascience.com/index.php/CJAS/article/view/287
Section
Animal Science
Author Biographies

Areadne Sosa

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana

Juana Galindo

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana

Ram?n Bocourt

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana

R. Rodr?guez

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana

Nereida Albelo

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana

A. Oramas

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, La Habana

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