Inclusion of Pichia guilliermondii on different culture media, on in vitro fermentation of Cynodon nlemfuensis
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Abstract
included the supernatant of each medium after centrifugation, and another treatment suspended again cell pellet in a buffer medium. A control without yeast was also included. An amount of 1 g of Cynodon nlemfuensis was incubated as fibrous substrate. Gas production was measured at 2, 4, 8, 12, 16, 20 and 24 h. Results showed that Levica
27 strain, cultured in malt extract broth and extract of yeast-peptoneglucose media, stimulated C. nlemfuensis gas production in a higher proportion (P < 0.001) than when it was cultured in a malt extract broth medium, which demonstrated the influence of metabolites thatproduce yeasts and their stimulating effect.
Key words: yeasts, rumen, additives
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