Clinical characteristics of induced estrus with hormones, and its relation with gestation percentage in nulliparous female water buffaloes

Carlos Gallego, Eunice Oba, Luis M. Fraga, R. García López, Mildred Méndez, E. Padrón


An amount of 82 nulliparous female water buffaloes were studied to compare the signs of induced estrus with hormone protocols (P). Treatments PI (n=29), PII (n=28) and PIII (n=25) received 2.0 mg of estradiol benzoate and intravaginal device of progesterone for nine days. After removing the device, 0.5 mg of prostaglandin F2α, 500 IU of equine chorionic gonadotropin and 0.5 mg of estradiol cypionate (PI and PII) were applied. In PIII, cypionate was replaced by 1000 IU of human chorionic gonadotropin after 48 h. Inseminations were carried out between 48-50 (PI), 56- 58 (PII) and 14-16 h (PIII) after the last hormone. A generalized
linear mixed model was applied, which considered the protocol as fixed effect, and the animal nested in the protocol as random effect. Tukey-Kramer test was applied for the multiple comparisons. The animals of PI showed higher swollen vulva (77.5 %) and higher pink coloration of the vaginal vestibule (75.2 %) than those from PII (16.7 and 16.26 %) and PIII (15.4 and 13.26%). Female buffaloes of PI (61.6 %) and PII (81.2 %) showed higher secretions than those of PIII (22.8 %). Uterine texcitement, tone cervical passage and insemination time showed no differences among protocols. Cervical passage showed a positive and high correlation with the gestation percentage. This last was negatively related to excitement and time during the insemination. It can be concluded that differences in the
expression of signs had no influence on the percentage of gestation among protocols.

Key words: female buffaloes, clinical signs, estrus, hormonal protocol

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