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completing three applications. Serum concentrations of Cu and Zn and bio-productive indicators were evaluated. Two groups with five cows were formed: one of them received C treatment and the other, used as control, was destined to evaluate hepatic reserves of Cu, Zn and Mn. C and D treatments increased significantly (P< 0.05) serum concentrations of Cu and Zn regarding the rest of them. C treatment increased significantly (P< 0.05) the hepatic levels of Zn and Cu, and Mn (P < 0.001). Reproductive indicators
were better (P<0.05) in cows that received C and D treatments, showing the highest levels of milk production (P< 0.05), after 60 d of lactation, regarding A and B groups. It can be concluded that C and D treatment were more effective to reestablish the organic levels of these microelements and favor productive and reproductive indicators of animals.
Key words: copper, zinc, manganese, doses, injectable, indicator, milking cows
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