Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis)

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Carlos Gallego
Margarita Pardo
Aline Camargos
D. Sousa
Mar?a E. Lenz
A. A. Ramos
A. M. Jorge
Fernanda da Cruz Ladim
Eunice Oba

Abstract

Twenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of
675 ? 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with
diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed
by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ? 0.75 mmol/L) and IGF-I (340 ? 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ? 0.12 mmol/L) and total proteins (58.4 ? 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period.
Key words: biochemical profile, follicular fluid, post-partum

Article Details

How to Cite
Gallego, C., Pardo, M., Camargos, A., Sousa, D., Lenz, M. E., Ramos, A. A., Jorge, A. M., da Cruz Ladim, F., & Oba, E. (2012). Glucose, cholesterol, total proteins and insulin-like growth factor type I values in the follicular fluid of female river buffaloes (Bubalus bubalis). Cuban Journal of Agricultural Science, 46(4). Retrieved from https://cjascience.com/index.php/CJAS/article/view/147
Section
Animal Science
Author Biographies

Carlos Gallego

Instituto de Ciencia Animal, Apartado Postal 24, San Jos? de las Lajas, Mayabeque, Cuba

Margarita Pardo

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

Aline Camargos

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

D. Sousa

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

Mar?a E. Lenz

Universidad Federal de Mato Grosso. Campo Grande. Mato Grosso. Brasil

A. A. Ramos

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

A. M. Jorge

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

Fernanda da Cruz Ladim

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

Eunice Oba

Universidad Estadual Paulista "Julio de Mesquita Filho" Campus de Botucat?. Brasil

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