Phytobiotic effect of mixed powder of medicinal plants on productivity and egg quality of laying hens

Main Article Content

R. Rodríguez
R. Aroche
Dalilis Donatién
Y. Martínez


To evaluate the phytobiotic effect of the mixed powder of medicinal plants on the productivity and egg quality of laying hens, 120 White Leghorn birds (Hybrid L-33), 27 weeks old, were used. For 10 weeks, the animals were placed according to a completely randomized design with three treatments, 10 repetitions per treatment and four birds per repetition. The experimental treatments consisted of a basal diet (T1) and the addition of 0.5 (T2) and 1.0 % (T3) of the mixed powder of leaves of medicinal plants (40 % of Anacardium  occidentale, 20 % of Psidium guajava, 20 % of Moringa oleifera and 20 % of Morinda citrifolia). T3 improved (P<0.05) laying intensity (80.18 to 85.53 %) and mass conversion (2.73 to 2.52) and reduced (P<0.05) the percentage of cracked eggs (0.22 to 0.08 %) in relation to the other experimental groups. However, diets did not change (P>0.05) the body weight of hens, egg weight, feed intake and broken, shell-less and dirty eggs, as well as external (shape index, weight, thickness, and surface area of the shell) and internal (albumen and yolk height, Haugh units and yolk color) quality of the egg of laying hens. Dietary addition of 1.0 % of mixed powder of medicinal plants (40 % A. occidentale, 20 % P. guajava, 20 % M. oleifera and 20 % M. citrifolia) is recommended to improve productive efficiency and reduce cracked eggs from laying hens.

Keywords: laying hen, medicinal powder, productive efficiency, final product

Article Details

How to Cite
Rodríguez, R., Aroche, R., Donatién, D., & Martínez, Y. (2023). Phytobiotic effect of mixed powder of medicinal plants on productivity and egg quality of laying hens. Cuban Journal of Agricultural Science, 57. Retrieved from
Animal Science


Aroche, R., Martínez, Y., Ruan, Z., Guan, G., Waititu, S., Nyachoti, C. M., Más, D. & Shile L. 2018. "Dietary inclusion of a mixed powder of medicinal plant leaves enhances the feed efficiency and immune function in broiler chickens". Journal of Chemistry, 2018(394): 1-6, ISSN: 2090-9071.

Burnett, E., Ishida, M., De Janon, S., Naushad, S., Duceppe, M.O., Gao, R. & Vinueza-Burgos, C. 2021. "Whole-genome sequencing reveals the presence of the bla CTX-M-65 Gene in Extended-Spectrum β-Lactamase-producing and multi-drug-resistant clones of Salmonella serovar Infantis isolated from broiler chicken environments in the Galapagos islands". Antibiotics, 10(3): 267, ISSN: 0021-8820.

Calvo-Brenes, P. & O´Hare, T. 2020. "Effect of freezing and cool storage on carotenoid content and quality of zeaxanthin-biofortified and standard yellow sweet-corn (Zea mays L.)". Journal of Food Composition and Analysis, 86(3): 103353, ISSN: 0889-1575.

Carter T.C. 1975. "The hen´s egg: Estimation of shell superficial area and egg volume using measurement of fresh egg weight and breadth alone or in combination". British Poultry Science, 16: 541-543, ISSN: 1466-1799.

Chen, H.L., Li, D.F., Chang, B.Y., Gong, L.M., Dai, J.G. & Yi, G.F. 2003. "Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers". Poultry Science, 82(3): 364-370, ISSN: 0032-5791.

Duncan, B. 1955. "Multiple ranges and multiple F test". Biometrics, 11(3): 1-42, ISSN: 1541-0420.

Evangelista, A.G., Corrêa, J.A.F., Pinto, A.C.S.M. & Luciano, F.B. 2022. "The impact of essential oils on antibiotic use in animal production regarding antimicrobial resistance–a review". Critical Reviews in Food Science and Nutrition, 62(19): 5267-5283, ISSN: 1549-7852.

Font, H., Noda, A., Torres, V., Herrera, M., Lizazo, D., Sarduy, L. & Rodríguez, L. 2007. “COMPARPRO: Comparación de Proporciones, Versión: 1.0”. Departamento de Biomatemática, Instituto de Ciencia Animal, San José de las Lajas, Mayabeque, Cuba.

Ghasemi, R., Zarei, M. & Torki, M. 2010. "Adding medicinal herbs including garlic (Allium sativum) and thyme (Thymus vulgaris) to diet of laying hens and evaluating productive performance and egg quality characteristics". American Journal of Animal and Veterinary Sciences, 5(2): 151-154, ISSN: 1557-4563.

Hidayat, R., Yunianto, V. D., Sukamto, B. & Sugiharto, S. 2021. "Effect of dietary supplementation of probiotic, phytobiotics or their combination on performance, blood indices and jejunal morphology of laying hens during post peak production". Online Journal of Animal and Feed Research, 11(1): 8-12, ISSN: 2228-7701.

Khan, S., Moore, R. J., Stanley, D. & Chousalkar, K.K. 2020. "The gut microbiota of laying hens and its manipulation with prebiotics and probiotics to enhance gut health and food safety". Applied and Environmental Microbiology, 86(13): e00600-20, ISSN: 0099-2240.

Kim, G.-B., Seo, Y.M., Kim, C.H. & Paik, I.K. 2011. "Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers". Poultry Science, 90: 75–82, ISSN: 0032-5791.

Kong, X. F., Hu, Y.L., Yin, Y.L., Wu, G.Y., Rui, R., Wang, D.Y. & Yang, C.B. 2006. "Chinese herbal ingredients are effective immune stimulators for chickens infected with the Newcastle disease virus". Poultry Science, 85(12): 2169-2175, ISSN: 0032-5791.

Martínez, Y., Escalona, A., Martínez, O., Olmo, C., Rodríguez, R., Iser, M., Betancur, C., Valdivié, M. & Liu, G. 2012. "The use of Anacardium occidentale as nutraceutical in hypoprotein diets for laying hens". Cuban Journal of Agricultural Science, 46(4): 395-401, ISSN: 2079-3480.

Martínez, Y., Rodríguez, R., Pupo, G.E., Rosabal, O., Olmo, C. & Valdivié, M. 2020. "Phytobiotic effect of Psidium guajava leaves powder on egg productivity and quality of laying hens". Cuban Journal of Agricultural Science, 54(4): 1-12, ISSN: 2079-3480.

Más, D., Martínez, Y., Rodríguez, B., Pupo, G., Rosabal, O. & Olmo, G. 2017. "Análisis preliminar de los metabolitos secundarios de polvos mixtos de hojas de plantas medicinales". Revista Cubana de Plantas Medicinales, 22(1): 1-9, ISSN: 1028-4796.

Más, D., Martínez, Y., Rodríguez, R., Betancur, C. & Rosabal, O. 2015. "Effect of dietary supplementation with Morinda citrifolia on productivity and egg quality of laying hens". Ciencia y Agricultura, 12(2): 7-12, ISSN: 0122-8420. http://dx.doi.10.19053/01228420.4348.

Mosayyeb, A., Mirghelenj, S.A., Hasanlou, P. & Shakouri Alishah, H. 2023. "Effects of pennyroyal (Mentha pulegium L.) supplementation on production performance, egg quality traits, and biochemical parameters of blood and egg in laying hens at later stages of the production period". Veterinary Medicine and Science, 9(1): 242-251, ISSN: 2053-1095.

Ni, H., Martínez, Y., Guan, G., Rodríguez, R., Más, D. & Liu, G. 2016. "Analysis of the impact of isoquinoline alkaloids, derived from Macleaya cordata extract, on the development and innate immune reaction in swine and poultry". BioMed Research International, 2016(11): 1-7, ISSN: 2314-6133.

Odunsi, A.A. 2003. Assessment of Lablab (Lablab purpureus) leaf meal as a feed ingredient and yolk colouring agent in the diet of layers. International Journal of Poultry Science, 2(1):71-74. ISSN: 1682-8356.

Olmo, C., Martínez, Y., Rodríguez, R., Rosabal, O., Pupo, G., Más, D., Donatién, D., Fonseca, R., Hidalgo, G. & Aguilar, N. 2013. Efecto promotor del polvo de hojas de Moringa oleifera en la producción y calidad del huevo de gallinas White Leghorn L-33. Taller de Generalización Avícola. Granma, Cuba.

Rabelo-Ruiz, M., Ariza-Romero, J. J., Zurita-González, M.J., Martín-Platero, A.M., Baños, A., Maqueda, M. & Peralta-Sánchez, J.M. 2021. "Allium-based phytobiotic enhances egg production in laying hens through microbial composition changes in ileum and cecum". Animals, 11(2): 448, ISSN: 2076-2615.

Rafeeq, M., Bilal, R.M., Batool, F., Yameen, K., Farag, M.R., Madkour, M. & Alagawany, M. 2023. "Application of herbs and their derivatives in broiler chickens: a review". World's Poultry Science Journal, 79(1): 1-23, ISSN: 0043-9339.

Rosabal, O., Martínez, Y., Rodríguez, R., Pupo, G., Olmo, C. & Más, D. 2017. "Efecto fitobiótico del polvo de hojas de Anacardium occidentale L. en las dietas de gallinas ponedoras". Revista Cubana de Plantas Medicinales, 22(1): 1-12, ISSN: 1028-4796.

Ruesga-Gutiérrez, E., Ruvalcaba-Gómez, J. M., Gómez-Godínez, L.J., Villagrán, Z., Gómez-Rodríguez, V.M., Heredia-Nava, D. & Arteaga-Garibay, R.I. 2022. "Allium-based phytobiotic for laying hens’ supplementation: effects on productivity, egg quality, and fecal microbiota". Microorganisms, 10(1): 117, ISSN: 2076-2607.

Salazar, I., Martínez, Y., Rodríguez, R., Olmo, C., Aroche, R., Pupo, G., Rosabal, O. & Más, D. 2017. "Efecto de la suplementación dietética con polvo mixto de plantas medicinales en la productividad y calidad del huevo de gallinas ponedoras". Revista de Producción Animal, 29(3): 1-5, ISSN: 2224-7920.

Saleh, A.A., Kirrella, A. A., Dawood, M. A. & Ebeid, T. A. 2019. "Effect of dietary inclusion of cumin seed oil on the performance, egg quality, immune response and ovarian development in laying hens under high ambient temperature". Journal of Animal Physiology and Animal Nutrition, 103(6): 1810-1817, ISSN: 1439-0396.

Savón, L., Scull, I. & Martínez, M. 2007. "Integral foliage meals of three tropical legumes for poultry feeding. Chemical composition, physical properties and phytochemical screening". Cuban Journal of Agricultural Science, 41(1): 359-341, ISSN: 2079-3480.

Sharma, M.K., White, D.L., Singh, A. K., Liu, H., Tan, Z., Peng, X. & Kim, W.K. 2022. "Effect of dietary supplementation of probiotic Aspergillus niger on performance and cecal microbiota in Hy-Line W-36 laying Hens". Animals, 12(18): 2406, ISSN: 2076-2615.

Torres, A., Sartor, C., Higgins, S., Wolfenden, A. D., Bielke, L., Pixley, C., Sutton, L., Tellez, G. & Hargis, B. 2005. " Effect of Aspergillus meal prebiotic (Fermacto) on performance of broiler chickens in the starter phase and fed low protein diets". The Journal of Applied Poultry Research, 14: 665–669, ISSN: 1537-0437.

UECAN (Unión de Empresas del Centro Avícola Nacional). 2007. Aportes de los piensos avícolas. Ed. Ministerio de la Agricultura. La Habana, Cuba.

Ullah, S., Ditta, Y.A., King, A. J., Pasha, T.N., Mahmud, A. & Majeed, K.A. 2022. "Varying isoleucine level to determine effects on performance, egg quality, serum biochemistry, and ileal protein digestibility in diets of young laying hens". PloS One, 17(1): e0261159, ISSN: 1932-6203.

Vlaicu, P.A., Panaite, T.D. & Turcu, R.P. 2021. "Enriching laying hens’ eggs by feeding diets with different fatty acid composition and antioxidants". Scientific Reports, 11(1): 20707, ISSN: 2045-2322.

Wasilewski, A., Zielińska, M., Storr, M. & Fichna, J. 2015. " Beneficial effects of probiotics, prebiotics, synbiotics, and psychobiotics in inflammatory bowel disease ". Inflammatory Bowel Diseases, 21(7): 1674-1682, ISSN: 1536-4844.

Most read articles by the same author(s)

1 2 3 4 > >>