In vitro antimicrobial and antioxidant activity of leaves and aqueous extract of four medicinal plants with phytobiotic potential in animal production

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R. Aroche
X. Jiang
R. Rodríguez
X. Li
Carolina Avellaneda
Y. Martínez


The aim of this study was to evaluate the in vitro antimicrobial and antioxidant properties of leaves and aqueous extracts from four medicinal plants (Anacardium occidentale, Psidium guajava, Morinda citrifolia, and Moringa oleifera). Six bacterial strains were used in the study, and the minimum bactericidal concentration (MBC) was determined for leaves, while the minimum inhibitory concentration (MIC) and MBC were evaluated for aqueous extracts. Additionally, the in vitro antioxidant activity was measured using 1,1-diphenyl-2-picryl-hydrazyl, and secondary metabolites were identified and quantified by ultra-high-performance liquid chromatography. Results showed that A. occidentale and P. guajava had the highest antimicrobial activity against all bacterial strains, with A. occidentale and P. guajava also demonstrating the highest antioxidant activity. A. occidentale had a high concentration of quercetin 3-O-glucoside-7-O-rhamnoside, kaempeferol-7-O-glucoside, quercetin, caffeic acid, and cinnamic acid in its leaves. In conclusion, A. occidentale and P. guajava are the most effective plants in terms of in vitro antimicrobial and antioxidant activity in their leaves and aqueous extracts, while Moringa oleifera has good antioxidant activity but no bactericidal effect, and Morinda citrifolia has no antimicrobial or antioxidant effect.

Keywords: antimicrobial activity, bactericidal, fine powder, leaves, secondary metabolite, antioxidant

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How to Cite
Aroche, R., Jiang, X., Rodríguez, R., Li, X., Avellaneda, C., & Martínez, Y. (2023). In vitro antimicrobial and antioxidant activity of leaves and aqueous extract of four medicinal plants with phytobiotic potential in animal production. Cuban Journal of Agricultural Science, 57. Retrieved from
Animal Science


Almeida, É.S., Oliveira, D. de & Hotza, D. 2019. "Properties and Applications of Morinda citrifolia (Noni): A Review". Comprehensive Reviews in Food Science and Food Safety, 18(4): 883–909, ISSN: 1541-4337.

Anand, G., Ravinanthan, M., Basaviah, R. & Shetty, V. 2015. "In vitro antimicrobial and cytotoxic effects of Anacardium occidentale and Mangifera indica in oral care". Journal of Pharmacy and Bioallied Sciences, 7(1): 69–74, ISSN: 0976-4879.

Aroche, R., Martínez, Y., Ayala, L., Rodríguez, R. & Rodríguez, Y. 2017. "Comportamiento productivo e incidencia de diarrea en cerdos posdestete suplementados con polvo mixto de hojas de plantas con propiedades nutracéuticas". Revista Ciencia y Agricultura, 14(2): 19–26, ISSN: 0122-8420.

Aroche, R., Martínez, Y., Ruan, Z., Guan, G., Waititu, S., Nyachoti, C. M., Más, D. & Lan, S. 2018. "Dietary Inclusion of a Mixed Powder of Medicinal Plant Leaves Enhances the Feed Efficiency and Immune Function in Broiler Chickens”. Journal of Chemistry, 2018: 1–6, ISSN: 2090-9063.

Bartlett, M. S. 1939. "A note of significance in multivariate analysis". Proceedings, Cambridge Philosophical Society, 35: 180–185.

Brito, E., Mendes, L., Garruti, D., Zocolo, G. & Lima, M. 2020. "Structure Astringency Relationship of Anacardic Acids from Cashew Apple (Anacardium occidentale L.)". ChemRxiv, ISSN: 2573-2293.

Ceballos-Francisco, D., Castillo, Y., De La Rosa, F., Vásquez, W., Reyes-Santiago, R., Cuello, A., Cuesta, A. & Esteban, M. Á. 2020. "Bactericidal effect on skin mucosa of dietary guava (Psidium guajava L.) leaves in hybrid tilapia (Oreochromis niloticus × O. mossambicus)". Journal of Ethnopharmacology, 259: 112838, ISSN: 0378-8741.

Chero-Nepo, D.A. & Ruiz-Barrueto, M.Á. 2016. "Efecto antibacteriano in vitro del extracto alcohólico de Psidium guajava (guayaba) y Medicago sativa (alfalfa) sobre Streptococcus mutans ATCC 25175". Revista de Salud & Vida Sipanense, 3(2): 6–12, ISSN: 2313-0369.

da Silva, R. A., Liberio, S. A., do Amaral, F. M. M., Fernandes do Nascimento, F. R., Brandão Torres, L. M., Monteiro Neto, V. & Meireles Guerra, R. N. 2016. "Antimicrobial and antioxidant activity of Anacardium occidentale L. flowers in comparison to bark and leaves extracts". Journal of Biosciences and Medicines, 4: 87–99, ISSN: 2327-5081.

Dhakad, A.K., Ikram, M., Sharma, S., Khan, S., Pandey, V.V. & Singh, A. 2019. "Biological, nutritional, and therapeutic significance of Moringa oleifera Lam". Phytotherapy Research, 33(11): 2870–2903, ISSN: 1099-1573.

Duncan, D. B. 1955. "Multiple range and multiple F tests". Biometrics, 11(1): 1-42, ISSN: 1541-0420.

Fang, F., Li, J., Pan, Q.-H. & Huang, W.-D. 2007. "Determination of red wine flavonoids by HPLC and effect of aging". Food Chemistry, 101(1): 428–433, ISSN: 0308-8146.

Feng, X., Wang, Z., Meng, D.-L. & Li, X. 2015. "Cytotoxic and antioxidant constituents from the leaves of Psidium guajava". Bioorganic & Medicinal Chemistry Letters, 25: 2193–2198, ISSN: 0968-0896.

Fieser, L. 2004. Experiments of organic chemistry. Reverté editorial, first edition, pp. 269–275.

Flores, G., Wu, S.-B., Negrin, A. & Kennelly, E. J. 2015. "Chemical composition and antioxidant activity of seven cultivars of guava (Psidium guajava) fruits". Food Chemistry, 170: 327–335, ISSN: 0308-8146.

Hernández, A., Pérez, J. M., Bosch, D. & Castro, N. 2019. "La clasificación de suelos de Cuba: énfasis en la versión de 2015". Cultivos Tropicales, 40(1): 31, ISSN: 0258-5936.

Karásková, K., Suchý, P. & Straková, E. 2015. "Current use of phytogenic feed additives in animal nutrition: a review". Czech Journal of Animal Science, 60(12): 521–530, ISSN: 1212-1819.

Kolmogorov, A. N. 1941. "On a logarithmic normal distribution law of the dimensions of particles under pulverization". Doklady Akademii Nauk Tadzhikskoi SSSR, 31(2): 99–101.

Martínez, Y., Martínez, O., Liu, G., Ren, W., Rodríguez, R., Fonseca, Y., Olmo, C., Isert, M., Aroche, R., Valdivié, M. & Nyachoti, C. M. 2013. "Effect of dietary supplementation with Anacardium occidentale on growth performance and immune and visceral organ weights in replacement laying pullets". Journal of Food, Agriculture and Environment, 11(3 & 4): 1352–1357, ISSN: 1459-0255.

Más, D., Martínez, Y., Rodríguez, R., Salazar, I., Aroche, R., López, B. & Marcella, D. 2016. "Efecto de la suplementación dietética con polvos de hojas de guayaba (Psidium guajava) y marañón (Anacardium occidentale) en el comportamiento productivo y la incidencia de diarrea en cerdos antes y después del destete". Revista Computadorizada de Producción Porcina, 23(2): 106–113, ISSN: 1026-9053.

Melo Menezes, K., Vieira Pereira, J., de Medeiros Nóbrega, D. R., Ramos de Freitas, A. F., Vieira Pereira, M. do S. & Vieira Pereira, A. 2014. "Antimicrobial and anti-adherent in vitro activity of tannins isolated from Anacardium occidentale Linn. (Cashew) on dental biofilm bacteria". Brazilian Research in Pediatric Dentistry and Integrated Clinic, 14(3): 191–198, ISSN: 1519-0501.

Mukhtar, H., Ansari, S., Ali, M., Naved, T. & Bhat, Z. 2004. "Effect of water extract of Psidium guajava leaves on alloxan-induced diabetic rats". Die Pharmazie, 59(9): 734–735, ISSN: 0031-7144.

Oanh, N. C., Thu, C. T. T., Hong, N. T., Giang, N. T. P., Hornick, J. L., & Dang, P. K. 2023. "Growth performance, meat quality, and blood characteristics of finisher crossbred pigs fed diets supplemented with different levels of green tea (Camellia sinensis) by-products". Veterinary World, 16(1), ISSN: 2231-0916.

Pandiselvi, P., Manohar, M., Thaila, M. & Sudha, A. 2019. Pharmacological activity of Morinda citrifolia L (Noni). In: Pharmacological benefits of natural products, First edition ed., India: JPS Scientific Publications, pp. 213–237, ISBN: 978-81-934054-2-0.

Ramírez, M. I., Dranguet, D. & Morales, J. A. 2020. "Actividad antiinflamatoria de plantas medicinales". Redel: Revista Granmense de Desarrollo Local, 16: 320–332, ISSN: 2664-3065.

Rios, J.L., Recio, M.C., Villar, A. 1988. "Screening methods for natural products with antimicrobial activity: A review of literature". Journal of Ethnopharmacology, 23(2-3): 127–149, ISSN: 0378-8741.

Roepke, J. & Bozzo, G. 2013. "Biocatalytic Synthesis of Quercetin 3-O-Glucoside-7-O-Rhamnoside by Metabolic Engineering of Escherichia coli". Chembiochem: European journal of chemical biology, 14(18): 2418–2422, ISSN: 1439-7633.

Salazar, I., Martínez, Y., Rodríguez, R., Olmo, C., Aroche, R., Pupo, G., Rosabal, O. & Más, D. 2017. "Efecto de la suplementación dietética con polvo mixto de plantas medicinales en la productividad y calidad del huevo de gallinas ponedoras". Revista de Producción Animal, 29(3): 1–5, ISSN: 2224-7920.

Salihu Abdallah, M., Nas, F. & Ali, M. 2019. "Antibacterial Activity of Psidium guajava Leaf and Stem Bark Extracts against Clinical Isolates of Staphylococcus aureus and Salmonella typhi". International Journal of Research in Pharmacy and Biosciences, 6(5): 11–17, ISSN: 2394-5893.

Senthilkumar, S., Deepa, K., Suganya, T., Janakarajan, M., Muralidharan, J. & Vasanthakumar, P. 2016. "Therapeutic properties of noni (Morinda citrifolia) and its products". International Journal of Science, Environment and Technology, 5(3): 1496–1502, ISSN: 2278-3687.

Shen, Q., Zhang, B., Xu, R., Wang, Y., Ding, X. & Li, P. 2010. "Antioxidant activity in vitro of selenium-contained protein from the Se-enriched Bifodobacterium animalis 01". Anaerobe, 16: 380–386, ISSN: 1075-9964.

Shih, M.C., Chang, C.M., Kang, S.M. & Tsai, M.L. 2011. "Effect of different parts (leaf, stem and stalk) and seasons (summer and winter) on the chemical compositions and antioxidant activity of Moringa oleifera". International Journal of Molecular Sciences, 12(9): 6077–6088, ISSN: 1422-0067.

Siddhuraju, P. & Becker, K. 2003. "Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves". Journal of Agricultural and Food Chemistry, 51(8): 2144–2155, ISSN: 0021-8561.

Singh, D., Sharma, S., Rani, R., Mishra, S. & Sharma, R. A. 2011. "Kaempferol-7-o-glucoside and their antimicrobial screening isolate from Cassia renigera wall". International Journal of Pharmaceutical and Clinical Research, 3(2): 30–34, ISSN: 0975-1556.

Siracusa, R., Fusco, R., Peritore, A. F., Cordaro, M., D’Amico, R., Genovese, T., Gugliandolo, E., Crupi, R., Smeriglio, A., Mandalari, G., Cuzzocrea, S., Di Paola, R. & Impellizzeri, D. 2020. "The Antioxidant and Anti-Inflammatory Properties of Anacardium occidentale L. Cashew Nuts in a Mouse Model of Colitis". Nutrients, 12(3): 834, ISSN: 2072-6643,

Skoufos, I., Bonos, E., Anastasiou, I., Tsinas, A., & Tzora, A. 2020. "Effects of phytobiotics in healthy or disease challenged animals". Feed Additives: aromatic plants and herbs in animal nutrition and health, 311-337.

Smirnov, N. 1948. "Table for estimating the goodness of fit of empirical distributions". Annals of mathematical statistics, 19(2): 297–281, ISSN: 2168-8990. http://doi.10.1214/amos/1177730256.

Souza, N. C., de Oliveira, J. M., Morrone, M. da S., Albanus, R. D., Amarante, M. do S. M., Camillo, C. da S., Langassner, S. M. Z., Gelain, D. P., Moreira, J. C. F., Dalmolin, R. J. S. & Pasquali, M. A. de B. 2017. "Antioxidant and Anti-Inflammatory Properties of Anacardium occidentale Leaf Extract". Evidence-Based Complementary and Alternative Medicine: eCAM, 2017: 8, ISSN: 1741-427X.

Sova, M. 2012. "Antioxidant and antimicrobial activities of cinnamic acid derivatives". Mini Reviews in Medicinal Chemistry, 12(8): 749–767, ISSN: 1875-5607.

Sunder, J., Sujatha, T. & Kundu, A. 2016. "Effect of Morinda citrifolia in growth, production and immunomodulatory properties in livestock and poultry: a review". Journal of Experimental Biology and Agricultural Sciences, 4(3S): 249–265, ISSN: 2320-8694.

Thorat, B., Kambale, A. & Patil, K. 2017. "Noni fruit crop is a versatile medicinal plant". Journal of Medicinal Plants Studies, 5(5): 247–249, ISSN: 2320-3862.

Valdivié, M., Martínez, Y., Mesa, O., Botello, A., Betancur, C. & Velázquez, B. 2020. "Review of Moringa oleifera as forage meal (leaves plus stems) intended for the feeding of non-ruminant animals". Animal Feed Science and Technology, 260(2020): 114338, ISSN: 0377-8401.

Wang, P., Chen, T., Sun, J., Xu, Q. & Zhang, Y. 2018. "Research Progress in the Application of Moringa oleifera in Animal Feed". Chinese Journal of Animal Nutrition, 30(7): 2488–2495, ISSN: 1006-267X.

Zhang, T., Zhang, B., Si, B., Zhang, N., Tu, Y., Zhou, C. & Diao, Q. 2018. "Effects of Moringa Leaf on Growth Performance, Slaughter Performance, Antioxidant Function and Meat Quality of Finishing Pigs". Chinese Journal of Animal Nutrition, 30(1): 255–261, ISSN: 1006-267X.

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