Stability and preservation of the probiotic additive with Lactobacillus pentosus LB-31 for animal production
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Abstract
The stability time and storage conditions of the probiotic additive with Lactobacillus pentosus LB-31 for animal production were determined. Completely random designs with six repetitions were used to monitor microbial viability for a month (0, 7, 14, 21 and 30 days) when the additive was stored under ambient conditions (24 ±2 ºC) and for six months (0, 30, 60, 90, 120 and 180 days) when stored under refrigeration (4±2 ºC). Cell concentration, pH and purity of the culture were also determined. The results showed that the lactic acid bacteria maintained its viability during the first 14 days of storage at room temperature. After this time, the microbial concentration decreased from 7.64 to 7.02 log cfu/mL (p=0.0028) and remained at 91 % viability until 30 days. Under refrigeration conditions, LB-31 was stably up to 60 days of storage with a concentration of 3.74x107 cfu/mL, and subsequently decreased to 106 cfu/mL (p<0.0001) with a viability of 78 % at the end of the study. Under both conditions, the pH decreased and the colonies maintain their morphological and cultural characteristics. It is concluded that the probiotic additive with Lactobacillus pentosus LB-31 without preservatives is stable for 14 days at room temperature (24±2 ºC), and for 60 days under refrigerated conditions (4±2 ºC).
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