Nutritional characterization of biofloc developed with water from the Ecuadorian Pacific for growing Litopenaeus vannamei

E. Ibarra, J. E. Llanes, Bárbara Rodríguez


The objective of this study was to determine the bromatological and amino acid composition of biofloc generated in the macrocosm tank for growing Litopenaeus vannamei with water from the Ecuadorian Pacific. Thalassisira sp. microalgae was inoculated to form the macrocosm (1.4 x 106 mL/L) and 40 shrimp/m2 with an average weight of 12.7 ± 1.2 g were placed, as well as commercial food with 35 % protein. To guarantee bacterial growth, a C:N ratio of 20:1 was maintained through the contribution of sugar cane molasses and nitrogen from the feed. The biofloc showed high protein levels (36.53 %), low lipid contribution (0.97 %) and high ash values
(33.39 %). There was presence of all amino acids, the highest levels were found for leucine, arginine, valine, threonine and phenylalanine. In contrast, tryptophan was the one with the lowest concentration and the lowest chemical score (0.56), resulting in the first limiting amino acid. Likewise, the isoleucine (0.70) and lysine (0.80) scores indicated that they were limiting amino acids of the biofloc that was produced. The index of biofloc essential amino acids was 0.89, which classifies as useful protein material. This study allows to conclude that the biofloc generated with Ecuadorian Pacific water presented an adequate protein quality, in terms of its amino acid composition, chemical score and essential amino acid index, so it can serve as a supplement to the balanced food for growing shrimp with biofloc technology.
Keywords: flocs, white shrimp, amino acids, bromatological composition

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