In vitro gas production of a ration for dairy cows supplemented with isolated yeasts from apple by-products

D. D?az-Plascencia; C. Rodr?guez-Muela; P. Mancillas-Flores; G. Corral; F. Salvador; L. Duran; Osmany La O

PDF

Published: Jun 1, 2013

D. D?az-Plascencia

C. Rodr?guez-Muela

P. Mancillas-Flores

G. Corral

F. Salvador

L. Duran

Osmany La O

Abstract

In order to assess the effect of adding yeasts isolated from apple by-products on the in vitro gas production of a ration for dairy cows, eight inocula were developed with the strains 2, 9, 11 and 13 of Kluyveromyces lactis (Kl); 3 and 8 of Issatchenkia orientalis (Io); 4 and 6 of Saccharomyces cerevisae (Sc). The in vitro gas production technique was applied for its assessment. The eight treatments consisted of 0.2 g of complete diet + 10 mL ruminal liquid + 20 mL artificial saliva and 1 mL of inoculum of the different strains. The inocula were evaluated in 120 glass flasks of 50 mL, with three repetitions per treatment and different sampling times: 12, 24 and 48 h for the variables ammoniac nitrogen (NH3), lactic acid (LA) and yeast counting (YC). For the gas production (PG), intervals of 3, 6, 12, 24 and 48h were established. The data were assessed with the procedure Proc Mixed of SAS 9.0, for a random design of eight treatments in plots divided in time. The results showed effect (P < 0.01) of strain and fermentation time in the variables NH3, LA and YC. The lowest values of NH3 were observed with the strain Kl-2, and the highest ones with the Io-3, Io-8 and Sc-6, with values of 22.5, 24.8, 24.8 and 24.1 mM/mL, respectively. Effect (P < 0.01) was found on the YC, with best performance of the strains Kl-2, Kl-9, Kl-11, Kl-13 and Sc-6, with cell counting of 1.9, 1.3, 1.2, 1.1 and 1.0 x 107cel/mL, respectively at 48h. It is concluded that the K. lactis yeast demonstrated to be the most effctive strain for ruminal fermentation, with better performance in respect to higher cell counting and reduction of the lactic acid.
Key words: yeasts, fermentation, gas production.

How to Cite

D?az-Plascencia, D., Rodr?guez-Muela, C., Mancillas-Flores, P., Corral, G., Salvador, F., Duran, L., & La O, O. (2013). In vitro gas production of a ration for dairy cows supplemented with isolated yeasts from apple by-products. Cuban Journal of Agricultural Science, 47(2). Retrieved from https://cjascience.com/index.php/CJAS/article/view/317

Issue

Vol. 47 No. 2 (2013): Cuban Journal of Agricultural Science

Section

Animal Science

Those authors that have publications with this journal accept the following terms:

1. They will retain their copyright and guarantee the journal the right of first publication of their work, which will be simultaneously subject to the License Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) that allows third parties to share the work whenever its author is indicated and its first publication this journal. Under this license the author will be free of:

Share — copy and redistribute the material in any medium or format
Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
NonCommercial — You may not use the material for commercial purposes.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

2. The authors may adopt other non-exclusive license agreements to distribute the published version of the work (e.g., deposit it in an institutional telematics file or publish it in a monographic volume) whenever the initial publication is indicated in this journal.

3. The authors are allowed and recommended disseminating their work through the Internet (e.g. in institutional telematics archives or on their website) before and during the submission process, which can produce interesting exchanges and increase the citations of the published work. (See the Effect of open access).

Author Biographies