Determination of fructans in vegetative organs of Agave Offoyana (Asparagaceae) and its potential use as a prebiotic
Article Sidebar
Main Article Content
Abstract
Fructans are carbohydrates in which the links between fructose residues predominate. They are synthesized in approximately 15 % of angiosperms. These molecules have various applications in food and health, due to the lack of digestibility and their selective use by the beneficial intestinal microbiota in monogastric animals. The objective of this study was to determine, using chromatographic methods, the presence of fructans in vegetative organs of the endemic species Agave offoyana (maguey). Plants were collected in three different physiological states during two climatic periods. They were taken from the managed floristic reserve Tres Ceibas de Clavellina, in Matanzas province, Cuba. Samples were analyzed by different chromatographic methods. Thin layer chromatography showed the presence of fructans in stems and leaves, but not in roots. The accumulation was greater in stems. Both organs contain from the trisaccharide to molecules with a high degree of polymerization. The presence of the polysaccharide was greater in the leaves at the base than in the middle and shoot apex. There was an increase in the accumulation of fructans in the organ samples collected during the dry season. High-performance liquid chromatography confirmed the presence of fructooligosaccharides extracted from the stem. Results showed that Agave offoyana accumulates fructans in the stem, although they are also present in leaves. This Cuban species can be used to obtain fructans for human and animal feeding and health.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Those authors that have publications with this journal accept the following terms:
1. They will retain their copyright and guarantee the journal the right of first publication of their work, which will be simultaneously subject to the License Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) that allows third parties to share the work whenever its author is indicated and its first publication this journal. Under this license the author will be free of:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
2. The authors may adopt other non-exclusive license agreements to distribute the published version of the work (e.g., deposit it in an institutional telematics file or publish it in a monographic volume) whenever the initial publication is indicated in this journal.
3. The authors are allowed and recommended disseminating their work through the Internet (e.g. in institutional telematics archives or on their website) before and during the submission process, which can produce interesting exchanges and increase the citations of the published work. (See the Effect of open access).
References
Aldrete-Herrera, P.I., López, M.G., Medina-Torres, L., Ragazzo-Sánchez, J.A., Calderón-Santoyo, M., González-Ávila, M. & Ortiz-Basurto, R.I. 2019. Physicochemical composition and apparent degree of polymerization of fructans in five wild Agave varieties: potential industrial use. Foods, 8(9): 404, ISSN: 2304-8158. https://doi.org/10.3390/foods8090404.
Alvarado-Loza, E., Orozco-Hernández, R., Ruíz-García, I., Paredes-Ibarra, F. & Fuentes-Hernández, V. 2017. The 2% of Agave inulin level in the rabbit feed affect spositively the digestibility and gutmicrobia. Abanico Veterinario, 7(3): 55-62, ISSN: 2448-6132. http://dx.doi.org/10.21929/abavet2017.73.6.
Ángeles-Espino, A., Dimas-Estrada, H.E., Ramírez-Alvarado, D., Cruz-Rubio, J.M., Palmeros-Suárez, P.A. & Gómez-Leyva, J.F. 2020. Molecular characterization of Agave tequilana mutants induced with gamma radiation Co60 and its effect in the fructooligosaccharides accumulation. Acta Universitaria, 30: 1-11, ISSN: 2007-9621. http://doi.org/10.15174/au.2020.2696.
Armas, R.R.A., Martínez, G.D. & Pérez, C.E.R. 2019. Inulin-typefructans: effect on gut microbiota, obesity and satiety. Gaceta Médica Espirituana, 21(2): 134-145, ISSN: 1608-8921.
Arrizon, J., Morel, S., Gschaedler, A. & Monsan, P. 2010. Comparison of the water-soluble carbohydrate composition and fructan structures of Agave tequilana plants of different ages. Food Chemistry, 122(1): 123-130, ISSN: 2590-1575. http://doi:10.1016/j.foodchem.2010.02.028.
Ayala, M.M.A., Hernández, S.D., Pinto, R.R., González, M.S.S., Bárcena, G.J.R., Hernández, M.O. & Torres, S.N. 2018. Prebiotic effect of two sources of inulin on in vitro growth of Lactobacillus salivarius and Enterococcus faecium. Revista Mexicana de Ciencias Pecuarias, 9(2): 346-361, ISSN: 2448-6698. http://dx.doi.org/10.22319/rmcp.v9i2.4488.
Bautista-Justo, M., García-Oropeza, L., Salcedo-Hernández, R. & Parra-Negrete, L.A. 2001. Azúcares en agaves (Agave tequilana Weber) cultivados en el estado de Guanajuato. Acta Universitaria, 11(1): 33-38, ISSN: 2007-9621. https://doi.org/10.15174/au.2001.325.
Chávez-Mora, I., Sánchez-Chiprés, D., Galindo-García, J., Ayala-Valdovinos, M.A., Duifhuis-Rivera, T. & Ly-Carmenatti, J. 2019. Efecto de oligofructosa de agave en dietas de gallinas ponedoras en la producción de huevos. Revista MVZ Córdoba, 24(1): 7108-7112, ISSN: 0122-0268. https://doi.org/10.21897/rmvz.1522.
de Lange, C.F.M., Pluske, J., Gong, J. & Nyachoti, C.M. 2010. Strategic use of feed ingredients and feed additives to stimulate gut health and development in young pigs. Livestock Science, 134(1-3): 124-134, ISSN: 1871-1413. https://doi:10.1016/j.livsci.2010.06.117.
de Zayas, A.A. 1980. Agave cajalbanensis: una nueva especie de Cuba occidental. Revista del Jardín Botánico Nacional, 1(2-3): 33-39, ISSN: 2410-5546.
Delgado, S.S.D.C., Abraham, J.M.J. & Simpson, J. 2012. Agave tequilana MADS genes show novel expression patterns in meristems, developing bulbils and floral organs. Sexual Plant Reproduction, 25(1): 11-26, ISSN: 0934-0882. https://doi.org/10.1007/s00497-011-0176-x.
Espinosa-Andrews, H., Urias-Silvas, J.E. & Morales-Hernández, N. 2021. The role of agave fructans in health and food applications: A review. Trends in Food Science & Technology, 114: 585-598, ISSN: 0924-2244. https://doi.org/10.1016/j.tifs.2021.06.022.
Ferrer-Serrano, C.M., Zumalacárregui-de-Cárdenas, B. & Mazorra-Mestre, M. 2023. Extracción de inulina a partir de piñas de desecho de henequeneras cubanas. Revista Cubana de Química, 35(2): 322-337, ISSN: 2224-5421.
Franco-Robles, E. & López, M. 2015. Implication of Fructans in Health: Immunomodulatory and Antioxidant Mechanisms. The Scientific World Journal, 2015: 15, ISSN: 2356-6140. http://dx.doi.org/10.1155/2015/289267.
García-Beltrán, J.A., Granado, P.L. & Bécquer, E.R. 2017. Las familias de las angiospermas de la flora de Cuba: visión diagnóstica desde los sistemas filogenéticos. Revista del Jardín Botánico Nacional, 38: 65-117, ISSN: 2410-5546.
García-Curbelo, Y., Ayala, L., Bocourt, R., Albelo, N., Nuñez, O., Rodríguez, Y. & López, M.G. 2018. Agavinas como prebióticos: su influencia en el metabolismo lipídico de cerdos. Cuban Journal of Agricultural Science, 52(4): 395-400, ISSN: 2079-3480.
García-Curbelo, Y., López, M.G. & Bocourt, R. 2009. Fructanos en Agave fourcroydes, potencialidades para su utilización en la alimentación animal. Revista Cubana de Ciencia Agrícola, 43(2): 175-177, ISSN: 0034-7485.
García-Curbelo, Y., López, M. G., Bocourt, R., Collado, E., Albelo, N. & Nuñez, O. 2015. Structural characterization of fructans from Agave fourcroydes (Lem.) with potential as prebiotic. Cuban Journal of Agricultural Science, 49(1): 75-80, ISSN: 2079-3480.
Godínez-Hernández, C.I., Aguirre-Rivera, J.R., Juárez-Flores, B.I., Ortiz-Pérez, M.D. & Becerra-Jiménez, J. 2016. Extraction and characterization of Agave salmiana Otto ex Salm-Dyckfructans. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 22(1): 59-72, ISSN: 2007-3828. https://doi:10.5154/r.rchscfa.2015.02.007.
Greuter, W. & Rankin, R. 2017. The Spermatophyta of Cuba. A Preliminary Checklist. Second, updated edition of the The Spermatophyta of Cuba with Pteridophyta added. Botanischer Garten and Botanisches Museum Berlin-Dahlem, Berlín.
Guillot, C.C. 2018. Update in prebiotics. Revista Cubana de Pediatría, 90(4): 648, ISSN: 1561-3119.
Hendry, G. 1993. Evolutionary origins and natural functions of fructans a climatological, biogeographic and mechanistic appraisal. New Phytologist, 123(1): 3-14, ISSN: 1469-8137. https://doi.org/10.1111/j.1469-8137.1993.tb04525.x.
Hernández, J. de J. 2018. El mezcal como patrimonio social: de indicaciones geográficas genéricas a denominaciones de origen regional. EmQuestão, 24(2): 404-433, ISSN: 1808-5245. http://dx.doi.org/10.19132/1808-5245242.404-433.
León, H. 1946. Flora de Cuba, Gimnospermas, Monocotiledóneas. Vol. I Contribuciones ocasionales del Museo de Historia Natural del Colegio La Salle No 8. La Habana. 441 pp.
Mancilla-Margalli, N.A. & López, M.G. 2006. Water-Soluble Carbohydrates and Fructan Structure Patterns from Agave and Dasylirion Species. Journal of Agricultural and Food Chemistry, 54(20): 7832-7839, ISSN: 1520-5118. https://doi.org/10.1021/jf060354v.
Márquez-López, R.E., Santiago-García, P.A. & López, M.G. 2022. Agave Fructans in Oaxaca’s Emblematic Specimens: Agave angustifolia Haw. and Agave potatorum Zucc. Plants, 11(14): 1834, ISSN: 2223-7747. https://doi.org/10.3390/plants11141834.
Mellado-Mojica, E., López-Medina, T.L. & López-Pérez, M.G. 2009. Developmental Variation in Agave tequilana Weber var. Azul Stem Carbohydrates. Journal of Agricultural and Food Chemistry, 3(1): 34-39, ISSN: 1749-0626.
Meyer, V.R. 2010. Practical High-Performance Liquid Chromatography. John Wiley and Sons, Ltd, New York, USA. pp. 5-141. ISBN: 978-0-470-68218-0.
Montañez-Soto, J., Venegas-González, J., Vivar-Vera, M. & Ramos-Ramírez, E. 2011. Extracción, caracterización y cuantificación de los fructanos contenidos en la cabeza y en las hojas del Agave tequilana Weber var. Azul. Bioagro, 23(3): 199-206, ISSN: 1316-3361.
ONEI. 2021. Anuario estadístico de Cuba 2020. Medio Ambiente. Oficina Nacional de Estadística e Información. Cuba. 60 pp.
Padilla-Camberos, E., Barragán-Álvarez, C.P., Díaz-Martínez, N.E., Rathod, V. & Flores-Fernández, J.M. 2018. Effects of Agave fructans (Agave tequilana Weber var. azul) on body fat and serum lipids in obesity. Plant Foods for Human Nutrition, 73: 34-39, ISSN: 1573-9104. https://doi.org/10.1007/s11130-018-0654-5.
Pérez, A.J., Calle, J.M., Simonet, A.M., Guerra, J.O., Stochmal, A. & Macías, F.A. 2013. Bioactive steroidal saponins from Agave offoyana flowers. Phytochemistry, 95: 298-307, ISSN: 0031-9422. https://doi.org/10.1016/j.phytochem.2013.06.020.
Pérez, A.J., Simonet, A.M., Calle, J.M., Pecio, L., Guerra, J.O., Stochmal, A. & Macías, F.A. 2014. Phytotoxic steroidal saponins from Agave offoyana leaves. Phytochemistry, 105: 92-100, ISSN: 0031-9422. https://doi.org/10.1016/j.phytochem.2014.05.014.
Pérez, F. & Martínez-Laborde, J.B. 1994. Utilización y transporte de los fotoasimilados. In: Pérez García, F. y J. B. Martínez-Laborde (eds.). Introducción a la Fisiología Vegetal. Ediciones Mundi-Prensa. Madrid, España. Pp. 87-94. ISBN: 84-7114-471-9.
Pérez-López, A.V., Simpson, J., Clench, M.R., Gomez-Vargas, A.D. & Ordaz-Ortiz, J.J. 2021. Localization and Composition of Fructans in Stem and Rhizome of Agave tequilana Weber var. Azul. Frontiers in Plant Science, 11: 1-16, ISSN: 1664-462X. https://doi.org/10.3389/fpls.2020.608850.
Plascencia, A., Gutiérrez-Mora, A., Rodríguez-Domínguez, M., Castañeda-Nava, J., Gallardo-Valdez, J. & Camacho-Ruíz, R.M. 2019. Characterization of fructans extracted from Agave mapisaga leaves. In: Gutierrez Mora, A. (ed.). Sustainable and Integrated use of Agave. CONACYT / CIATEJ / Agared, Zapopan, Jalisco, Mexico. Pp. 101-106. ISBN: 978-607-8734-03-0.
Regalado, E., Godínez-Hernández, C.I., Aguirre Rivera, J.R., Camacho, R.M. & Juárez, B.I. 2021. Characterization of fructans from stems of Agave salmiana Otto ex Salm-Dyck and A. tequilana F.A.C. Weber at full physiological maturity. Botanical Sciences, 9(2): 388-397, ISSN: 2007-4476. https://doi.org/10.17129/botsci.2641.
Ritsema, T. & Smeekensm, S. 2003. Engineering fructan metabolism in plants. Journal of Plant Physiology, 160: 811-820, ISSN: 1618-1328. https://doi.org/10.1078/0176-1617-01029.
Romero-Jiménez, M., Castañeda-Noa, I. & de las Mercedes, L. 2015. Origen y estado actual de la flora espermatófita en áreas naturales de cayo Las Brujas, Villa Clara. Revista del Jardín Botánico Nacional, 36: 31-46, ISSN: 2410-5546.
Taiz, L. & Zeiger, E. 2002. Respiration and Lipid Metabolism. In: Taiz, L. y E. Zeiger (eds.). Plant physiology. Sinauer Associates Inc. Sunderland, Massachusetts, USA. Pp. 245-247. ISBN: 0-87893-823-0.
Trujillo, T.L.E., Gómez, R.R., Banguela, C.A., Soto, R.M., Arrieta, S.J.G. & Hernández, G.L. 2004. Catalytical properties of N-glycosylated Gluconacetobacterdiazotrophicus levansucrase produced in yeast. Electronic Journal of Biotechnology, 7(2): 115-123, ISSN: 0717-3458. https://doi.org/10.4067/S0717-34582004000200005.
Trujillo-Ramírez, D., Bustos-Vázquez, M.G., Martínez-Velasco, A. & Torres-de los Santos, R. 2023. Integral use of Henequen (Agave fourcroydes): applications and trends-a review. Tropical and Subtropical Agroecosystems, 26(2): 1-18, ISSN: 1870-0462. http://doi.org/10.56369/tsaes.4619.
Van den Ende, W. 2013. Multifunctional fructans and raffinose family oligosaccharides. Frontiers in Plant Science, 4: 1-11, ISSN: 1664-462X. https://doi.org/10.3389/fpls.2013.00247.
Verma, D.K., Patel, A.R., Thakur, M., Singh, S., Tripathy, S., Srivastav, P.P. & Aguilar, C.N. 2021. A review of the composition and toxicology of fructans, and their applications in foods and health. Journal of Food Composition and Analysis, 99: 103884, ISSN: 1096-0481. https://doi.org/10.1016/j.jfca.2021.103884.
Vijn, I., van Dijken, A., Lüscher, M., Bos, A., Smeets, E., Weisbeek, P., Wiemken, A. & Smeekens, S. 1998. Cloning of sucrose: sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose. Plant Physiology, 117(4): 1507-1513, ISSN: 1532-2548. https://doi.org/10.1104/pp.117.4.1507.
Wack, M. & Blaschek, W. 2006. Determination of the structure and degree of polymerisation of fructans from Echinacea purpurea roots. Carbohydrate Research, 341(9): 1147-1153, ISSN: 1873-426X. https://doi.org/10.1016/j.carres.2006.03.034.
Wang, L., Yang, H., Huang, H., Zhang, C., Zuo, H.X., Xu, P., Niu, Y.M. & Wu, S.S. 2019. Inulin‑type fructans supplementation improves glycemic control for the prediabetes and type 2 diabetes populations: results from a GRADE‑assessed systematic review and dose-response meta‑analysis of 33 randomized controlled trials. Journal of Translational Medicine, 17(410): 1-19, ISSN: 1479-5876. https://doi.org/10.1186/s12967-019-02159-0.
Waterhouse, A.L. & Chatterton, N.J. 1993. Glossary of fructan terms. In: Michio Suzuki, N. y J. Chatterton (eds.). Science and technology of fructans. CRC Press. Boca Raton, USA. Pp. 1-7. ISBN: 0-8493-5111-1.