Application of an in vitro methodology for detecting active saponins in plant extracts. Technical note
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Abstract
randomized experimental design was applied, with five extracts per treatment (legume) and three repetitions per extract. The average value of the three repetitions per extract of each legume was considered the experimental unit. The enterolobium extracts were positive to both tests, while the other three legumes were negative (P < 0.0001). With the use of this methodology, based on the combination of these two tests, the presence of active saponins in E. cyclocarpum extracts was confirmed. Moreover, it could state that in the extracts of acacia, albizia and leucaena there were not presence of these compounds or tannins able to hydrolyzing the membrane of red blood cells.
Key words: saponins, foam test, blood agar test, tropical legumes
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