Evaluation of polyethylene glycol phosphate aqueous two-phase systems in purifying pepsin from residues of the slaughter house
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Abstract
The pepsin distribution in both homogenates showed similar performance to the pattern enzyme and distribution constants higher than the unit (PH 2.7 and BH 1.5) were obtained. The purification indicators for both preparations showed a purification factor higher than 3(HP 3.25 and BH 3.81) and yields higher than 60 % (PH 74.16 and BH 61. 54). The system formed by PEG 1500/phosphate at a distribution temperature of 20 ºC was the best variant. This method of separation and purification of pepsin is a simple and rapid alternative, also allows obtaining an adequate purity for further extractives steps of the enzyme.
Key words: enzymes, purification systems, homogenates
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