Isolation and identification of strains of Bacillus spp. in different ecosystems, with probiotic purposes, and their use in animals

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Published: Mar 9, 2016

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Grethel Milián
Universidad de Matanzas “Camilo Cienfuegos”, Autopista Varadero km 3 ½, Matanzas, Cuba
A. J. Rondón
Universidad de Matanzas “Camilo Cienfuegos”, Autopista Varadero km 3 ½, Matanzas, Cuba
M. Pérez
Universidad de Matanzas “Camilo Cienfuegos”, Autopista Varadero km 3 ½, Matanzas, Cuba
L. M. Samaniego
Universidad de Matanzas “Camilo Cienfuegos”, Autopista Varadero km 3 ½, Matanzas, Cuba
J. Riaño
Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, E 24071, León, España
Raúl Bocourt
Instituto de Ciencia Animal, Apartado Postal 24, San José de las Lajas, Mayabeque, Cuba
R. Ranilla
Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, E 24071, León, España
M. D. Carro
Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, E 24071, León, España
M. Rodríguez
Universidad de Matanzas “Camilo Cienfuegos”, Autopista Varadero km 3 ½, Matanzas, Cuba
M. Laurencio
Universidad de Matanzas “Camilo Cienfuegos”, Autopista Varadero km 3 ½, Matanzas, Cuba

Abstract

Strains of Bacillus spp., taken from different ecosystems, were isolated and identified. These ecosystems were: fresh and old bovine feces, content of chicken caecum, decayed tomato juice, and a mixture of soil and bovine blood. They were used for producing probiotic biological preparations, which can be used in livestock interest. The strains were previously selected, considering the morphological characteristics of the genre Bacillus spp. and their resistance to the main natural barriers of digesta flow (acid pH 1, 2 and 3, and biliary salts 1.5 g L-1 of Ox-Bilis). For identifying strains, different methods were used: traditional methods, fermentation of carbohydrates (API 50 CHB) and polymerase chain reaction (PCR). A total of 100 strains were obtained, 48 expressed characteristics of Bacillus spp. genre, and only three showed resistance to biliary salts. The strains corresponded to Bacillus subtilis, sub species subtilis, which is one of the most used for producing nutritional additives with probiotic effect on livestock hinterest.

Key words: isolation, identification, Bacillus spp., nutritional additives

Article Details

How to Cite
Milián, G., Rondón, A. J., Pérez, M., Samaniego, L. M., Riaño, J., Bocourt, R., Ranilla, R., Carro, M. D., Rodríguez, M., & Laurencio, M. (2016). Isolation and identification of strains of Bacillus spp. in different ecosystems, with probiotic purposes, and their use in animals. Cuban Journal of Agricultural Science, 48(4). Retrieved from https://cjascience.com/index.php/CJAS/article/view/562
Issue
Vol. 48 No. 4 (2014): Cuban Journal of Agricultural Science
Section
Animal Science

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