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Stability and preservation of the probiotic additive with Lactobacillus pentosus LB-31 for animal production Sosa Cossio, Dailyn García Hernández, Yaneisy Dustet Mendoza, Julio C. Sosa Ceijas, Areadne García Curbelo, Yanelys García Hernández, Yoleisy Albelo Dorta, Nereyda animal feeding bacteria preservation alimentación animal bacteria preservación The stability time and storage conditions of the probiotic additive with Lactobacillus pentosus LB-31 for animal production were determined. Completely random designs with six repetitions were used to monitor microbial viability for a month (0, 7, 14, 21 and 30 days) when the additive was stored under ambient conditions (24 ±2 ºC) and for six months (0, 30, 60, 90, 120 and 180 days) when stored under refrigeration (4±2 ºC). Cell concentration, pH and purity of the culture were also determined. The results showed that the lactic acid bacteria maintained its viability during the first 14 days of storage at room temperature. After this time, the microbial concentration decreased from 7.64 to 7.02 log cfu/mL (p=0.0028) and remained at 91 % viability until 30 days. Under refrigeration conditions, LB-31 was stably up to 60 days of storage with a concentration of 3.74x107 cfu/mL, and subsequently decreased to 106 cfu/mL (p<0.0001) with a viability of 78 % at the end of the study. Under both conditions, the pH decreased and the colonies maintain their morphological and cultural characteristics. It is concluded that the probiotic additive with Lactobacillus pentosus LB-31 without preservatives is stable for 14 days at room temperature (24±2 ºC), and for 60 days under refrigerated conditions (4±2 ºC). Se determinó el tiempo de estabilidad y condiciones de almacenamiento del aditivo probiótico con Lactobacillus pentosus LB-31 destinado a la producción animal. Se utilizaron diseños completamente aleatorizados con seis repeticiones para monitorear la viabilidad microbiana durante un mes (0, 7, 14, 21 y 30 días) al conservar el aditivo en condiciones ambientales (24 ±2 ºC) y durante seis meses (0, 30, 60, 90, 120 y 180 días) cuando se almacenó en refrigeración (4±2 ºC). Se determinó, además, la concentración celular, el pH y la pureza del cultivo. Los resultados mostraron que la bacteria ácido láctica mantuvo su viabilidad durante los primeros 14 días de conservación a temperatura ambiente. Después de este tiempo, la concentración microbiana disminuyó de 7.64 a 7.02 log ufc/mL (p=0.0028) y se mantuvo en 91 % de viabilidad hasta los 30 días. En condiciones de refrigeración, LB-31 se comportó estable hasta los 60 días de conservación con una concentración de 3.74x107 ufc/mL, y posteriormente disminuyó a 106 ufc/mL (p<0.0001) con una viabilidad de 78 % al finalizar el estudio. En ambas condiciones, el pH disminuyó y se comprobó que las colonias mantenían sus características morfológicas y culturales. Se concluye que el aditivo probiótico con Lactobacillus pentosus LB-31 sin conservantes es estable durante 14 días a temperatura ambiente (24±2 ºC), y por 60 días en condiciones refrigeradas (4±2 ºC). Instituto de Ciencia Animal 2025-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf application/pdf text/html application/epub+zip text/xml https://cjascience.com/index.php/CJAS/article/view/1155 Cuban Journal of Agricultural Science; Vol. 58 (2024): January-December; https://cu-id.com/1996/v58e19 Cuban Journal of Agricultural Science; Vol. 58 (2024): January-December; https://cu-id.com/1996/v58e19 2079-3480 0864-0408 eng spa https://cjascience.com/index.php/CJAS/article/view/1155/1716 https://cjascience.com/index.php/CJAS/article/view/1155/1753 https://cjascience.com/index.php/CJAS/article/view/1155/1717 https://cjascience.com/index.php/CJAS/article/view/1155/1718 https://cjascience.com/index.php/CJAS/article/view/1155/1719 Copyright (c) 2025 Licencia CC Reconocimiento-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0