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Biofertilization with Gluconacetobacter diazotrophicus and Funneliformis mosseae in Guinea grass (Megathyrsus maximus cv. Likoni). Technical note González, P. J. Ramírez, J. F. Reyes, R. Rivera, R. An experiment was carried out under semi-controlled conditions to evaluate the effect of biofertilization with Gluconacetobacter diazotrophicus and Funneliformis mosseae on Guinea grass (Megathyrsus maximus cv. Likoni). Five treatments (simple and combined inoculation with G. diazotrophicus and F. mosseae, fertilization with 25 mg N kg-1 of soil and a control without biofertilizers or nitrogenous fertilizer) were studied in a completely random design with five repetitions. Biofertilization with G. diazotrophicus and F. mosseae separately produced higher effects than the control without biofertilizers. However, with the combined application of both, the highest frequency of mycorrhizal colonization was reached (52.4 %), as well as concentrations of N in aerial biomass (18.5 g kg-1) and aerial biomass yields (7.45 g pot-1) and radical (7.38 g pot-1), similar to those obtained with nitrogen fertilization. It is concluded that the co-inoculation with G. diazotrophicus and F. mosseae is effective in improving nitrogenous nutrition and biomass yields of Megathyrsus maximus cv. Likoni.Key words: rhizobacteria, arbuscular mycorrhizae, nitrogen, growth Instituto de Ciencia Animal 2022-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html application/epub+zip application/zip https://cjascience.com/index.php/CJAS/article/view/1059 Cuban Journal of Agricultural Science; Vol. 56 No. 3 (2022): Cuban Journal of Agricultural Science Cuban Journal of Agricultural Science; Vol. 56 Núm. 3 (2022): Cuban Journal of Agricultural Science 2079-3480 0864-0408 eng https://cjascience.com/index.php/CJAS/article/view/1059/1419 https://cjascience.com/index.php/CJAS/article/view/1059/1506 https://cjascience.com/index.php/CJAS/article/view/1059/1509 https://cjascience.com/index.php/CJAS/article/view/1059/1510 Copyright (c) 2022 Cuban Journal of Agricultural Science