Selection of rhizobia isolates from nodules of the forage legume Pueraria phaseoloides (tropical kudzu)

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Ionel Hern?ndez
Mar?a C. N?poles
P. R. Rosales
R. Ba?os
J. F. Ram?rez

Abstract

Ten rhizobia isolates were used for selecting those showing potentialities for the preparation of a biofertilizer allowing a better establishment
of tropical kudzu in livestock production soils. Their possible taxonomic distribution and their capacity of growing in six carbon sources and produce polyhydroxybutyrates, were determined. Also, in vitro nodulation bioessays were conducted in the host plant. A completely randomized design with ten observations per treatment was used. All isolates resulted possible members of the Bradyrhizobium genus. Six of them consumed all the sugars as only carbon source, galactose was the least consumed.? Polyhydroxybutyrate concentrations produced by these isolates were between 0.001 and 0.009 g.L-1, what was significantly higher than the rest of the isolates. Plants inoculated with two of the isolates showed greater aerial dry mass which tallies with those having greater effective nodulation. From the ten isolates, four showed greater potentialities for the preparation of an effective inoculant for tropical kudzu culture.
Key words: identification, biofertilizer, sugars, PHB, nodulation

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How to Cite
Hern?ndez, I., N?poles, M. C., Rosales, P. R., Ba?os, R., & Ram?rez, J. F. (2013). Selection of rhizobia isolates from nodules of the forage legume Pueraria phaseoloides (tropical kudzu). Cuban Journal of Agricultural Science, 47(3). Retrieved from https://cjascience.com/index.php/CJAS/article/view/360
Section
Pasture Science and other Crops
Author Biographies

Ionel Hern?ndez

Instituto Nacional de Ciencias Agr?colas (INCA). Gaveta Postal No.1. San Jos? de las Lajas, Mayabeque. Cuba. CP 32700

Mar?a C. N?poles

Instituto Nacional de Ciencias Agr?colas (INCA). Gaveta Postal No.1. San Jos? de las Lajas, Mayabeque. Cuba. CP 32700

P. R. Rosales

Instituto Nacional de Ciencias Agr?colas (INCA). Gaveta Postal No.1. San Jos? de las Lajas, Mayabeque. Cuba. CP 32700

R. Ba?os

Instituto de Investigaciones de Pastos y Forrajes. Avenida Independencia. km 8 ?. Boyeros, Ciudad de La Habana. Cuba

J. F. Ram?rez

Estaci?n Experimental de Pastos y Forrajes de Villa Clara. Instituto de Investigaciones de Pastos y Forrajes (IIPF) ?Finca Palo Bonito?. Santo Domingo, Villa Clara. Cuba

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